![]() Can also initialize from previously-added PCA parameters-this means you can compute PCA on a predicate sample, copy the PCA vectors to other samples, and initialize SNEe on those identically-computed vectors for greater cross-sample similarity.Can initialize the tSNE algorithm with previously-computed LDA parameters.(Together, these two improvements allow tSNE to be run on one million events with 12 parameters! Note that this can take two hours on a laptop.).Within each revision are listed the new features (major and minor), and any bug fixes. The changes are listed in reverse chronological order. Comments, concerns and questions regarding it should be addressed to her.Listed below are the changes made to FlowJo since the release of FlowJo, version 6.4, on November 16th 2005. Jinghang Zhang is solely responsible for the content of this site. Partec (flow cytometers, antibodies, etc.) Miltenyi Biotec (magnetic antibody bead separation instrumentation and associated antibodies) Jackson ImmunoResearch Laboratories (quality secondary antibodies) Invitrogen (Molecular Probes/Caltag/Qdots/CloneTech) Innova Biosciences (antibody labeling kits) Paul Robinsonīeckman Coulter (flow cytometers, antibodies, etc.)īecton Dickinson/Pharmingen (flow cytometers, antibodies, etc.)ĭakoCytomation (flow cytometers, antibodies, etc.) WinMDI Tutorial - by Gérald Gergori, Kathy Ragheb and J. Titering Antibodies - by Carlton and Sigrid Stewart Tissue Dissociation Guide - by Worthington Biochemical Corporation Technical Center for those using secondary and tertiary antibodies: and click the 'Technical Center' tab. Simultaneous analysis of DNA content and surface immunophenotype using gentle ethanol fixation techniques - by Bill Telford, Louis King and Pamela Fraker Protocols on the web including flow cytometry ![]() Alternatively, a short form will allow you direct access to the registration page (your name and email are required). This is a great gift to the community by Molecular Probes detection technologies from Invitrogen. (published by John Wiley & Sons), by Howard Shapiro, can be accessed free of charge! While you can't print the materials, you can read whatever you want. Synchronization Wizard movie tutorial - used to analyze data with perturbed populations Intracellular cytokine protocols - by Joost Schuitemaker ![]() G-force calculator for your centrifuge - it's the G-force that's important, not the RPMs! Multiparametric Analysis of Apoptosis by Flow and Image Cytometry - by William Telford, Akira Komoriya and Beverly PackardĬell Cycle basics - from University College LondonĬell Cycle protocols and analysis - University of Texas MD Anderson Cancer CenterĬonjugation of monoclonal antibodies - also by Mario Roederer, PhDĬytometry Techniques and Protocols (Purdue)ĭiva Quick Start Guide - from Becton Dickinsonįlow Cytometry on the Web - Salk Institute - We're rated 5 th!!! Washington State University College of Veterinary MedicineĪpoptosis - University of Texas MD Anderson Cancer CenterĬaspases - includes many links to important sites. NIH Nonhuman Primate Reagent Resource - hosted by Beth Israel Deaconess Medical Center Since the initial publication of the work, 770 pages of content is new (43%) and revised (57%) on average every year! Current Protocols in Immunology covers the latest in antibody detection and preparation, molecular immunology, innate immunity, tumor immunology, engineering immune molecules and receptors, and more as well as animal models for analyses offers valuable reference information in five extensive appendices, including commonly used reagents, equipment and techniques and a full appendix devoted to the CD system of leukocyte surface molecules. Updated every three months in all formats, Current Protocols in Immunology is constantly evolving to keep pace with the very latest discoveries and developments. ◆ Current Protocols in Immunology, also available online through AECOM's library!Ĭurrent Protocols in Immunology is also a "best-practices" collection that provides comprehensive coverage of immunological methods from classic to the most cutting edge: the most sophisticated protocols in immunology today. On average, 52% of content is new and 48% has been revised every year since initial publication. Updated every three months in all formats, Current Protocols in Cytometry is constantly evolving to keep pace with the very latest discoveries and developments. It is the most complete set of peer-reviewed protocols for flow and image cytometry available. ◆ Current Protocols in Cytometry, available online through AECOM's library!Ĭurrent Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide.
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